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Image Search Results
Journal: bioRxiv
Article Title: Generation of an optimised Cumate toolkit for tuneable protein expression during in vitro and in vivo studies of Burkholderia cenocepacia
doi: 10.1101/2025.11.26.690688
Figure Lengend Snippet: Cumate induction allows inducible control of protein expression from B. cenocepacia within THP-1 cells . A) Gentamicin protection assays demonstrate comparable bacterial burden, assessed as CFU/ml, within THP-1 cells under induced and non-induced conditions as compared to the MH1K strain. B) Immunoblot of sfGFP expression after 2, 4, 6, and 24 hours of cumate induction within THP-1 cells reveals detectible levels of sfGFP after 2 hours of induction. C) Immunofluorescence microscopy of internalised MH1K and MH1K containing CymR GV -sfGFP strains within THP-1 cells under induced and uninduced conditions reveal co-localisation of sfGFP and B. cenocepacia in an induction dependent manner. DNA has been stained with Hoechst 33342, B. cenocepacia with Alexa Fluor 568, sfGFP visualised through intrinsic fluorescence, and actin filaments labelled with SiR-actin 647.
Article Snippet:
Techniques: Control, Expressing, Western Blot, Immunofluorescence, Microscopy, Staining, Fluorescence